Abstract:

 To explore the effects of goat endometrial cells and sex hormones on secretion content of VEGF and IFN-γ in uNK cells. Using immortalized goat endometrial stromal cells (ESC) and epithelial cells (EEC) as an in vitro research model, the effects of the endometrial cells and sex hormones (E₂, P₄) on secretion content of VEGF and IFN-γ in uNK cells was detected by ELISA assay. When uNK cells co-cultured with EEC, estrogen alone or together with P₄ significantly inhibited the secretion levels of IFN-γ and VEGF in uNK cells (P<0.05), while the P₄ alone had no significant effect. When uNK cells co-cultured with ESC, P₄ alone or together with E₂ can significantly enhance the secretion of IFN-γ in uNK cells (P<0.05), whereas E₂ alone or together with P₄ significantly inhibited the secretion level of VEGF (P<0.05). These results indicated that the sex hormones play a role in regulatoring secretion of uNK cells co-cultured with EEC or ESC.